Rapid UHPLC Analysis of Reduced Monoclonal Antibodies using an Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300SB-C8 Column Authors
نویسندگان
چکیده
An Agilent ZORBAX sub-2 μm Rapid Resolution High Definition (RRHD) 300SB-C8 reversed-phase column was used under optimized chromatographic conditions for delivering highly resolved ultra fast separations of reduced and alkylated monoclonal antibodies. The StableBond C8 phase and RRHD column technology, in combination with robust gradient elution conditions enabled efficient, fast, and effective separation of light chain and two heavy chain monoclonal antibody variants in under 4 minutes. Additionally, alternate mobile phase compositions were employed, making separations flexible for ultra fast LC/MS analysis. At elevated operating pressures and temperature, the stable C8 phase and rugged column packing technology delivered highly reproducible separations and delivered excellent chromatographic run-to-run results.
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